Qiang Cai;Zhenqiang Wang;Shouhua Wang;Mingzhe Weng;Di Zhou;Chen Li;Jiandong Wang;Erzhen Chen;Zhiwei Quan
期刊论文
https://doi.org/10.1098/rsob.160247
SCIE
Open Biology
7#1
2017/2/1
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Long non-coding RNA LINC00152 had been reported as an oncogene in gastric and hepatocellular cancer. In this study, we show that LINC00152 is overexpressed in gallbladder cancer (GBC) tissue samples and cell lines. The high LINC00152 levels correlated negatively with the overall survival time in GBC.patients. Functionally, LINC00152 dramatically promoted cell migration,invasion and epithelial–mesenchymal transition (EMT) progression in vitro. In vivo, LINC00152 overexpression significantly promoted tumour peritoneal spreading and metastasis. Mechanistic analyses indicated that LINC00152 functions as a molecular sponge for miR-138, which directly suppresses the expression of hypoxia inducible factor-1a (HIF-1a). We revealed that miR138 is a suppressor of GBC cell metastasis and EMT progression, and a similar phenomenon was observed in HIF-1a knockdown NOZ cells. Through binding to miR-138, LINC00152 has an oncogenic effect on GBC. Overall, our study suggested that the LINC00152/miR-138/HIF-1a pathway potentiates the progression of GBC, and LINC00152 may be a novel therapeutic target.
抑制LncRNA-VSTM4介导的异常自噬在逆转胆囊癌化疗耐药中的机制研究